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API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.

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A Gram-stain and oxidase test should be performed prior to set-up of tests. Refer to the Table of Reactions below for the substrates contained in each well, the specific reaction principle, and colour changes. Thomas, Veterinary Microbiology. Citrate utilization citrate is the only source of carbon.

Inoculation The wells of individual substrate sets can be exposed by cutting the end tag of the sealing strip and slowly peeling it back.

All systems should be read after 48 hours for the identification of Miscellaneous Gram-negative bacilli. Hydrolysis of gelatin is indicated by dispersal of the black particles throughout the well. This causes a pH rise as indicated by bromothymol blue. Prolonged incubation, insufficient incubation, improper filling of wells, or inadequate inoculum may lead to false results. Materials Required but not provided The following materials may be required but are not provided: These can be 20ee individually or as a set 20w ordering product code MB Production of indolepyruvate by deamination of tryptophan.



Citrate is the sole carbon source, which if utilized results in a pH rise, indicated by bromothymol blue, with a colour change from green to blue. Remove the strips or tray from the incubator, peel back the sealing tape.

Using the results obtained, the indices of the positive reactions are circled. Strips stored in this manner must be used within 7 days.

Refer to the procedure chart for a condensed version of the gaoerie procedures. Argine dihydrolase converts arginine into ornithine, ammonia and carbon dioxide. When Actinobacillus or Pasteurella sp. Biochemical Characterisics of Enterotoxigenic Aeromonas sp. Yellow-green is indicative of a negative result. Bromothymol blue indicates formation of the specific amine cadaverine.

MBA Microbact 12E 20 pouches, identifications.

API®/ID32 | bioMérieux Industry website

Using a sterile pipette or dropper bottle, overlay glerie substrates underlined on the holding tray with sterile mineral oil, i. Test can be evaluated immediately after the addition of the reagent.

The 12B strips can be 2e alongside the 12E, but in a separate tray. Miscellaneous Gram-negative bacilli – Weakly positive reactions are recorded as negative results. Indole Kovacs reagent forms a pink-red complex with indole.

Motility and DNase tests are recommended for further speciation of this group. The inclusion of a 12B strip is strongly advised.

Record the results under the appropriate heading on the report form.



The reactions are evaluated as positive or negative by comparing them with the colour chart. Evaluate 15 to 30 minutes after the addition of reagents.

Production of a red colour within a few minutes of the addition of the reagent indicates that nitrate reduction to nitrite NO 2 has occurred.

Some bacterial strains may have atypical biochemical reactions due to unusual nutritional requirements or mutations and may be difficult to identify.

BIOMIC V3 Microbiology System – Automated API Reader – Giles Scientific USA

This will determine whether nitrate has been reduced completely to nitrogen gas N2. Once the foil pouch has been opened, unused strips must be placed back in the foil pouch, galrie the foil pouch taped closed. All organisms belonging to the family Enterobacteriaceae reduce nitrates to nitrites and give a positive reaction. The 12B strip can be used in conjunction with the 12A strip for ap identification of oxidase-positive, nitrate-negative, and glucose-nonfermenters MGNB as well as the Enterobacteriaceae.

If further speciation is required for Yersinia spp. Green reactions occurring at 48 hours should be interpreted as negative.

Well 8 Indole production – add 2 drops of Indole Kovacs reagent. Warning and precautions 1.